Transcriptome analysis of intestinal tissues from QIH-induced mice

This experiment was performed to investigate transcriptomic changes in intestinal tissues during and after QIH in mice. Small pieces of intestinal tissue were collected from QIH-induced mice at multiple time points: before QIH induction (0 h), during the QIH state (18 h and 36 h), and after recovery (168 h). RNA was extracted using the PureLink RNA Mini (Thermo Fischer Scientific). mRNA was enriched using the NEBNext Poly(A) mRNA Magnetic Isolation Module, and sequencing libraries were prepared using the NEBNext Ultra II RNA Library Prep Kit for Illumina. Library concentration and quality were assessed using the Qubit dsDNA HS Assay Kit and Agilent 2000 Bioanalyzer (Agilent Technologies, Inc., Santa Clara, CA). Paired-end sequencing (2 Ã? 150 bp) was performed on a HiSeq platform (Azenta Life Sciences).