PGRMC2 is a pressure-volume regulator critical for myocardial responses to stress in mice

Progesterone receptors are classified into nuclear and membrane-bound receptor families.  In this study, we evaluate the role of progesterone receptor membrane-bound component-2 (PGRMC2) in the heart. We use a heart-specific knockout (KO) mouse model (MyH6•Pgrmc2flox/flox) in which the Pgrmc2 gene was specifically deleted in cardiomyocytes. Here we show that PGRMC2 serves as a mediator of steroid hormones for rapid calcium signaling in cardiomyocytes to maintain cardiac contraction and support stroke volume and cardiac output by regulating the cardiac pressure-volume relationship. The KO hearts from both male and female mice exhibit impairment in regulating the pressure-volume relationship.  Under hypoxic conditions, this dysregulation in the KO hearts is subsequently progressed to congestive left and right ventricular failure., Total mRNA transcriptome sequencing of heart samples was studied using the NovaSeq X Plus platform (Illumina)., , # PGRMC2 is a pressure-volume regulator critical for myocardial responses to stress in mice

## Descriptions

Comparing mRNA sequencing from mouse heart tissues in three wild-type (WT) and three PGRMC2 knockout (KO) samples.

Sample:

• WT.1, WT.2, WT.3, KO.1, KO.2, KO.3.
• WT stands for Wild-Type (control group).
• KO stands for Knockout (PGRMC2).
• The species being studied is Mus musculus (mouse)

FASTQ Entry data description:

1st Line (Identifier)

• LH00626 → The High-Throughput sequencing identification Tag number
• 19 → Run number.
• 22GHMLLT4 → Flow cell ID.
• 5 → Lane number.
• 1101 → Tile number on the flow cell.
• 12313:1056 → X and Y coordinates of the cluster on the tile.
• 1:N:0:ATGTGATT+AAGTAACG
o 1 → Read number (1 for forward read, 2 for reverse read in paired-end sequencing).
o N → Indicates if this read failed (Y) or passed (N) quality filters.
o 0 → Index number (likely a sample barcode).
o ATGTGATT+AAGTAACG → Dual index adapters used for multiplexing.

2nd Line...