Microarray gene expression from hepatitis B virus infection to hepatocellular carcinoma. Microarray gene expression from hepatitis B virus infection to hepatocellular carcinoma

Background: The pathogenesis of hepatitis B virus (HBV)-caused hepatocellular carcinoma (HCC) is complex and not fully understood. In clinical, the effective prevention and treatment of HCC rely on the accurate diagnosis. We developed a biology network approach to investigate the potential mechanisms and biomarkers of each stages from HBV infection to HCC. Methods Global gene profiling of healthy individuals (HC), HBV carriers (HBVC), chronic hepatitis B patients (CHB), liver cirrhosis (LC) and HCC was analyzed by gene array. Differentially expressed genes (DEG) were found by RVM (Random variance model) corrective ANOVA and STC (Series Test of Cluster) analysis. Overall design: peripheral blood mononuclear cells (PBMCs) from 3 healthy individuals (HC),3 HBV carriers (HBVC), 3 chronic hepatitis B patients (CHB), 3 liver cirrhosis (LC) and 3hepatocellular carcinoma (HCC) samples