Proteomic analyses to determine the impact of anti-FASII on Staphylococcus aureus protein expression in low stress conditions.

The associated study shows that Staphylococcus aureus can adapt to fatty acid synthesis inhibitors by incorporating exogenous fatty acids in their phospholipids. Our results show that bacteria adapt in low stress medium more readily than in high stress medium. We compared two environments, i- high membrane stress and ii- low membrane stress, and followed responses of strain USA300-FPR3757 spa to the biocide triclosan. High membrane stress medium contains free fatty acids. Low membrane stress medium is supplemented with serum. Strains are grown in BHI medium ("BHI"), BHI containing 3 fatty acids C14, C16, and C18:1 ("FA"), FA medium with the biocide triclosan ("FA-Tric"), FA medium supplemented with serum ("SerFA"), and SerFA supplemented with triclosan (SerFA-Tric). BHI, FA, and SFA cultures are harvested at OD600=1. FA-Tric and SerFA-Tric cultures are harvested at 2 hours and 4 hours. The data show protein reads corresponding to each growth condition.

本研究显示，金黄色葡萄球菌能够通过将外源性脂肪酸整合到其磷脂中，适应脂肪酸合成抑制剂。我们的结果表明，细菌在低应激介质中的适应性相较于高应激介质更为显著。我们比较了两种环境：一是高膜应激环境，二是低膜应激环境，并追踪了美国300-FPR3757 spa 菌株对消毒剂三氯生（triclosan）的响应。高膜应激介质含有游离脂肪酸；低膜应激介质则添加了血清。菌株在BHI培养基（“BHI”）中培养，BHI培养基含有三种脂肪酸C14、C16和C18:1（“FA”），FA培养基含有消毒剂三氯生（“FA-Tric”），FA培养基补充了血清（“SerFA”），以及添加了三氯生的SerFA（SerFA-Tric）。BHI、FA和SFA培养液在OD600=1时收获。FA-Tric和SerFA-Tric培养液在2小时和4小时时收获。数据显示，与每种生长条件相对应的蛋白质读数。