We have measured absolute copy numbers and dynamics of cohesin, CTCF and their regulators NIPBL, WAPL and sororin by mass spectrometry, fluorescence-correlation spectroscopy and fluorescence recovery

NIAID Data Ecosystem40

Synthetic biology meets proteomics: Construction of à la carte QconCATs for absolute protein quantification

蛋白质绝对定量

QconCAT组装

We report a new approach to the assembly and construction of QconCATs, quantitative concatamers for proteomic applications that yield stoichiometric quantities of sets of stable isotope-labelled inter

NIAID Data Ecosystem20

FastCAT accelerates absolute quantification of proteins by using multiple short non-purified chimeric standards

蛋白质绝对定量

神经学分析

Absolute (molar) quantification of proteins provides the analytical rationale for system-level modelling of diverse molecular mechanisms. FastCAT method employs multiple short (<50 kDa) stable-isotope

DataCite Commons2022-04-04 更新60

Quantitative Phosphoproteomic Analysis of the Tumor Necrosis Factor Pathway

磷酸化蛋白质组学

TNF信号通路分析

Protein phosphorylation has become a focus of many proteomic studies due to the central role that it plays in biology. We combine peptide-based gel-free isoelectric focusing and immobilized metal affi

NIAID Data Ecosystem30

Absolute quantification of TNF-RSC members from isolated TNF-RSC from A549 cells

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