Patient stratification by hierarchical clustering analysis of actionable gene expression.

Each row and column means an individual gene and an individual patient sample, respectively. After categorization by BCLC stages, the patients were ordered by Euclidean distance and linkage according to the ratios of 2−ÄCt values in each tumor compared to the cognate non-tumor. In the heat map, the red and green color reflect high and low expression levels, respectively, as depicted in the scale bar at the bottom. The scale represents the gene expression ratios from 4 to −4 in fold difference of 2−ÄCt values. (A) Hierarchical clustering of 5 actionable genes in HCC patients. (B) Hierarchical clustering of VEGFR2 and PDGFRβ. (C) Hierarchical clustering of VEGFR2 and FGFR1. (D) Hierarchical clustering of VEGFR2, PDGFRβ, and EGFR. (E) Hierarchical clustering of VEGFR2, PDGFRβ, and mTOR.