Identification by in-gel trypsinolysis and tandem mass-spectrometry of phosphorylated sites within human fetal pTaucoex in E. coli.

Numbering for two alternatively spliced Tau isoforms is shown. Likelihood that the identified phosphopeptide (underlined) serves as a 14-3-3-binding site is estimated from “-”(low probability) to “+++” (high probability), compared to the existing literature (right column). Sites of trypsinolysis is designated by “.”.