Gene edition in haematopoietic cell lines. PCR-based strategy for introducing CRISPR/Cas9 machinery into hematopoietic cell lines

Due to the difficult transfection of blood cells, we developed an alternative strategy to generate CRISPR constructs. Our constructs were easily introduced into hard-to-transfect leukemic cells. Gene edition efficiencies obtained by Endonuclease T7-EI and restriction fragment length polymorphism were confirmed by sanger sequencing and next generation sequencing.