RNA-seq for blood Ly6Chi and Ly6Clo monocyte in WT and RBP-Jfl/fl Lyz2-Cre mice

To evaluate the consequences of RBP-J loss of function in blood monocyte subsets, we sequenced RNA of blood Ly6Chi monocyte and Ly6Clo monocyte from WT and RBP-Jfl/fl Lyz2-Cre mice. Overall design: Blood Ly6Chi and Ly6Clo monocytes were isolated from WT and RBP-Jfl/fl Lyz2-Cre mice, and total RNA was extracted using total RNA purification kit. RNA was converted into RNA-seq quantification libraries, and RNA-seq libraries were sequenced with the pair-end option.