mRNA-encoded fluorescent proteins enable superior organelle labeling for live cell imaging

Live cell labeling of various organelles is of great demand in the research field of cell biology. However, current approaches often lack an optimal balance between efficiency and versatility. We took the advantages of chemical modified mRNA to express various organelle located fluorescent proteins. This approach facilitated highly efficient multi-organelle labeling across diverse cell types, both in vitro and in vivo. Notably, employing these tools revealed distinctive behaviors of mitochondria, endoplasmic reticulum, and peroxisomes during spike protein-mediated cell fusion. Thus, our results demonstrate the potency of mRNA-encoded fluorescent proteins as powerful tools for advancing biological research.