Supplementary Tables S1-S5 from Interplay between RNA interference and heat shock response systems in <i>Drosophila melanogaster</i>

The genome expression pattern is strongly modified during the heat shock response (HSR) to form adaptive state. This may be partly achieved by modulating microRNA levels that control the expression of a great number of genes that are embedded within the gene circuitry. Here, we investigated the cross-talk between two highly conserved and universal house-keeping systems, the HSR and microRNA machinery, in <i>Drosophila melanogaster</i>. We demonstrated that pronounced interstrain differences in the microRNA levels are alleviated after heat shock (HS) to form a uniform microRNA pattern. However, individual strains exhibit different patterns of microRNA expression during the course of recovery. Importantly, HS-regulated microRNAs may target functionally similar HS-responsive genes involved in the HSR. Despite the observed general downregulation of primary microRNA precursor expression as well as core microRNA pathway genes after HS, the levels of many mature microRNAs are upregulated. This indicates that the regulation of miRNA expression after HS occurs at transcriptional and post-transcriptional levels. It was also shown that deletion of all <i>hsp70</i> genes had no significant effect on microRNA biogenesis but might influence the dynamics of microRNA expression during the HSR.

基因组表达谱在热激反应（heat shock response, HSR）过程中发生显著重塑，以形成适应性状态。这一过程可部分通过调控微小RNA（microRNA）的水平实现，后者可调控嵌入基因调控网络中的大量基因的表达。本研究以黑腹果蝇（*Drosophila melanogaster*）为研究对象，探究了两种高度保守且普遍存在的持家系统——热激反应与微小RNA调控系统之间的交叉对话。研究发现，不同品系间微小RNA水平的显著差异在热激（heat shock, HS）后得以缓解，形成统一的微小RNA表达谱。然而，在恢复阶段，不同品系的微小RNA表达模式仍存在差异。值得注意的是，受热激调控的微小RNA可靶向参与热激反应的功能相似的热激响应基因。尽管观测到热激后微小RNA初级前体以及微小RNA通路核心基因的表达普遍下调，但众多成熟微小RNA的水平却呈现上调。这表明热激后微小RNA的表达调控同时发生在转录水平与转录后水平。研究同时证实，敲除所有热激蛋白70（hsp70）基因对微小RNA的生物发生无显著影响，但可能会影响热激反应过程中微小RNA表达的动态变化。

搜集汇总

背景与挑战

背景概述

该数据集包含5个补充表格（S1-S5），源自一项研究果蝇中RNA干扰与热休克响应系统相互作用的工作。研究聚焦于热休克前后microRNA表达模式的变化，揭示了microRNA在转录和转录后水平的调控机制，并探讨了hsp70基因删除对microRNA表达动态的影响。