Watermelon 'Arka Manik' In vitro seedling endophytic bacterial diversity analysis

This study was aimed at assessing the endophytic bacterial diversity associated with the in vitro grown seedlings derived from surface-disinfected seed embryos to study the bacterial transmission from embryos to seedlings adopting 16S rRNA taxonomic profiling. The DNA extracted from the tissue-homogenate from the four samples was taken through 16S rRNA V3-V4 region amplicon profiling. The findings indicated the transmission of diverse bacteria in a cultivation recalcitrant form to seedlings with differntial distribution profiles in different parts of seedlings.