Data underlying the publication: Preserving Full Spectrum Information in Imaging Mass Spectrometry Data Reduction

<strong>FT-ICR</strong>Human kidney tissue was surgically removed during a fullnephrectomy, and remnant tissue was processed for researchpurposes by the Cooperative Human Tissue Network (CHTN)at Vanderbilt University Medical Center. Human biospecimenswere collected in compliance with the CHTN protocols,institutional IRB policies, and the National Cancer Institute’sbest practices for procurement of remnant surgical researchmaterial. The tissue was flash-frozen over an isopentane-dryice slurry and embedded in carboxymethylcellulose. Tissuesections were cryosectioned with a thickness of 10 μm andthaw-mounted onto indium tin-oxide-coated glass slides (DeltaTechnologies, Loveland, CO, USA). 1,5-Diaminonaphthalene(DAN) was applied to the tissue surface using a TM Sprayer M3(HTX Technologies, Chapel Hill, NC, USA). The sample wasimaged (50 μm pitch) directly after matrix application witha 15T MALDI Fourier-transform ion cyclotron resonance (FT-ICR) mass spectrometer (Solarix, Bruker Daltonics, Billerica,MD, USA). Briefly, data were generated from m/z 300-2000with a 4M file size in negative ion mode.<br><strong>qTOF</strong>C57BL6/J mice were retro-orbitally infected with <em>S. aureus</em> Newman and sacrificed humanely five days post-infection. Kidneys were flash frozen on an isopentane/dry ice slurry and embedded in 2.6\% carboxymethylcellulose. 5 μm thick sections were collected using a Leica Biosystems CM3050S cryostat and thaw-mounted onto indium tin oxide coated glass slides. Sections were washed with cold 150 mM ammonium formate for 45 seconds for three total washes. 5 mg of 4-(dimethylamino)cinnamic acid matrix was applied using an in-house sublimation device. MALDI IMS data were collected in negative ion mode from m/z 400-2000 using a Bruker MALDI timsTOF fleX platform with a 5 μm step size, 25% laser power, and 25 shots per pixel.