Supplemental Material for Riles and Fay, 2018

Data availability statement: All strains and constructs
are available upon request to the corresponding author. Supplemental Table 1: Strains used in this study. Supplemental
Table 2: Recombinant strain barcode indices, sequencing and phenotypes used for
QTL mapping. Supplemental Table 3: Primers used in this study. Supplemental
Table 4: Summary of candidate genes tested for two QTL regions. Supplemental
Table 5: Genes tested for complementation using a MoBY and/or a hemizygosity
test. Supplemental Table 6: Colony size measurements using ImageJ for HN6 X KO
Hemizygotes on Chromosome 14. Supplementary Figure 1: Ethanol and heat
sensitivity of control strains. Supplementary Figure 2: Logarithm of the odds
ratio (LOD) of a quantitative trait locus for heat and ethanol tolerance at
high temperature across the genome. Supplementary Figure 3: Protein alignment
of HN6, Oak and S288c for SEC24 and PSD1. Supplementary Figure 4: Phenotypes of resistant wild yeast
strains, Oak, Wine and S288c, containing high copy Oak allele plasmids grown at
40o. Data File S1. Genotypes and phenotypes of recombinant strains
used for QTL mapping. The raw data used to call genotypes for QTL mapping has
been deposited into NCBI's SRA under BioProject: PRJNA480857.