Differentially expressed miRNAs identified in various influenza virus infection

To further identified miRNAs of BALB/c mouse lungs infected with A/Anhui/1/2013 (H7N9) (hereafter referred to as H7N9/AH1-PB2-627K(WT)) and mutant variants with PB2 amino acid substitutions (avian-like H7N9/AH1-PB2-627E and mammalian-adapted H7N9/AH1-PB2-627E/701N) , miRNA microarray was carried out. Balb/c mice were inoculated with 5x105 EID50 influenza A virus, and lungs were collected on day 3 and 5 post-infection. Then total RNA was extracted and RNA integrity were then assessed for miRNA microarray experiment.