Heat measured, oxygen consumed and ATP synthesized by BAT mitochondria.

Conditions were as described under methods. Values are average ±S.E., of the number of experiments shown in parentheses. Each experiment was performed with different mitochondria preparations. The differences of heat released measured without and with Ca2+ in both uncoupled (a) and coupled (b) mitochondria were statistically significant (p<0.0005). For respiration, (c)p<0.025 and (d)p<0.050. For ATP synthesis, (e)p<0.0025 in uncoupled and non significant in coupled mitochondria (f).