Absolute and relative quantification of 16S rRNA, <i>nirK</i> and <i>nirS</i> genes in both estuarine sediment types (HBM and LBM).

Analyses was performed in triplicate for each biological replicate (n = 3). Gene copy numbers given were corrected for PCR inhibition. a. Detection limit, 101 copies; PCR efficiency, 90.4%; standard curve R2, 0.99; inhibition factor range: 0.07–0.10. b. Detection limit, 101 copies; PCR efficiency, 87%; standard curve R2, 0.99; inhibition factor range: 0.36–0.62. c. Detection limit, 101 copies; PCR efficiency, 62.9%; standard curve R2, 0.99; inhibition factor range: 0.31–0.55. Absolute and relative quantification of 16S rRNA, nirK and nirS genes in both estuarine sediment types (HBM and LBM).