20240903 Hamm & Gray spot plate images

E. coli strains MG1655, MJG0224 (MG1655 ∆ppk-749), MJG0226 (MG1655 ∆relA782), MJG0344 (MG1655 ∆rpoS746), MJG1116 (MG1655 ∆ppk-749 ∆relA782), MJG1119 (MG1655 ∆ppk-749 ∆rpoS746), MJG1136 (MG1655 ∆relA782 spoT207::cat+), MJG1137 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+), MJG1205 (MG1655 rpoB3449), MJG1234 (MG1655 ∆ppk-749 rpoB3449), MJG1235 (MG1655 ∆relA782 rpoB3449), MJG1236 (MG1655 ∆ppk-749 ∆relA782 rpoB3449), MJG1237 (MG1655 ∆relA782 spoT207::cat+ rpoB3449), MJG1241 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ rpoB3449), MJG1605 (MG1655 ∆relA782 spoT207::cat+ / pUC18 (bla+)), MJG1606 (MG1655 ∆relA782 spoT207::cat+ / pSPOT1 (spoT+, bla+)), MJG1608 (MG1655 ∆relA782 spoT207::cat+ / pSPOT3 (spoT D259N, bla+)), MJG1613 (MG1655 ∆relA782 spoT207::cat+ / pSPOT4 (spoT D73N D259N, bla+)), MJG1306 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pUC18 (bla+)), MJG1307 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pPPK8 (ppk+ bla+)), MJG1308 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT1 (spoT+ bla+)), MJG1609 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT2 (spoT D73N bla+)), MJG1610 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT3 (spoT D259N bla+)), or MJG1614 (MG1655 ∆ppk-749 ∆relA782 spoT207::cat+ / pSPOT4 (spoT D73N D259N bla+)) were grown overnight at 37C with shaking in LB broth, then rinsed twice with PBS and normalized to A600 = 1.5-µl spots of 10-fold serial dilutions were plated on LB agar and on M9 agar containing 0.4% (w/v) glucose with or without 0.05% casamino acids (Fisher Scientific cat. #BP1424), 1% tryptone (Fisher Scientific cat. #BP1421), or 0.5% yeast extract (Becton Dickinson cat. #288620). Plates were incubated overnight at 30 or 37C, as indicated.