five

Mouse kidney mitochondria LC-MS/MS

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https://www.omicsdi.org/dataset/pride/PXD000233
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ApoE KO mice were treated with Mas receptor agonist AVE 0991 fo 16 weeks. At the age of 6 months mice were killed, the kidneys were dissected and the mitochondria were isolated by differential centrifugation. Next, 2DE electrophoresis was conduced. Healthy control (C57BL/6J mice), apoE KO and apoE KO treated with AVE 0991 were compared. Gel pieces containing protein spots of interest were destained, reduced, alkylated, digested with modified trypsin and analysed by LC-MS/MS method.
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2016-05-09
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