Anole ocular patterns of gene expression

For the purpose of facilitating gene annotation, a transcriptome profile of the developing anole eye was used to identify coding and non-coding regions in the Anolis genome. RNA datasets were generated by collecting samples from three subregions of the anole posterior globe (central, temporal, and nasal) which was comprised of nerual retina, retinal pigmented epithelium, choriod, and scleral tissues. A total of five sample replicates were made. Each replicate incorporated pooled tissues collected from three stage 16.5 developing anole embryos. Five RNA-seq datasets of three different subregions of the anole posterior globe (central, temporal, and nasal) were generated using embryos of mixed sexes collected at stage 16.5 that were obtained from random populations of wild-caught anoles that originated from south Florida.