Epigenomic and transcriptomic features induced by silencing of ZNF280C in colon cancer cells and knock-out of Zfp280c in mice [ChIP-Seq, ATAC-Seq]

Znic finger proteins play crucial roles in development and disease, including tumorigenesis. Here, we identifed ZNF280C as a novel oncogenic driver in colorectal cancer, and systematically studied the molecular mechanisms underlying ZNF280C-mediated malignant transformation and progression. RKO colon cancer cells stably expressing a ZNF280C-targeting shRNA controlled by a tetracycline-inducible promoter were treated with or without 1 ug/ml doxycycline for 72 hours, and the cells were harvested for analysis as described below. For transgenic mice experiments, Zfp280c knock-out or wild-type C57BL/6J mice were sacrificed at 12 weeks of age and colon tissues were havested for transriptomic analysis as described below.