Gene expression data from study on the role of DISC1 607 variants on immune responses against Toxoplasma gondii

A major mental illness-related susceptibility factor, Disrupted in schizophrenia (DISC1), is involved in altered host immune responses against Toxoplasma gondii (T. gondii) infection. Specifically, our gene expression studies have revealed that DISC1 Leu607Phe variation, which changes DISC1 interaction with activating transcription factor 4 (ATF4), modifies gene expression patterns upon T. gondii infection. EBV-transformed human lymphoblastoid cells with DISC1 607 Leu/Leu and Phe/Phe genotypes were infected with T. gondii for 48 hours. Cells were harvested and total RNA was isolated by Qiagen RNEasy kit. Genee expression profiling was conducted with Illumina HT-12 v4.0 platform at the Microarray Core Facility at the Sidney Kimmel Comprehensive Cancer Center at Johns Hopkins University.