Early-life thermal stress mediates long-term alterations in hypothalamic microglia [RRBS]

We identified the microglial population in the chick anterior hypothalamus (AH) and showed that early-life thermal stress (on day 3 post-hatch) influences the inflammatory process in the AH. We described alterations in genome-wide CpG methylation profile of hypothalamic microglia between heat-conditioned and non-conditioned 10-day-old chicks prior LPS challenge. We suppose that changes in CpG DNA methylation might be involved in reprogramming microglial function. Microglial total CpG-methylation profile of 10-day-old heat-conditioned and non-conditioned chicks before LPS-challenge by reduced representation bisulfite sequencing (RRBS)