The CRISPR/Cas9 system has been widely applied for plant genome editing. The commonly used SpCas9 has been shown to rely on the protospacer adjacent motif (PAM) sequences in the canonical form NGG and
To identify potential off-target mutations generated by the NRT1.1 genome editing system, Illumina deep sequencing on T0 seedling leaf tissue from event 1 was performed. This sequencing run produced 7
Raw data. This file contains raw data with individual data points or replicates for Figs. 1, 2, 4, 7 and Additional file 1 Figure S3, S4, S5, S8, S10, S11. (XLSX 120 kb)