A single cell RNA-Seq study of splenic marginal zone lymphomas (SMZL)

Purpose: Single cell RNA-Seq was used to characterize in depth splenic samples obtained from splenic marginal zone lymphomas Methods: 3 frozen samples of spleen from SMZL patients were selected and thawed for performing a 10X genomics single cell RNA-Sequencing 3' assay. Results: Tumor cells formed mainly patient specific-B cell clusters, while T-cells from the 3 patients clustered together. Conclusion: Single RNA-Seq unveils the cellular composition of splenic tumors from SMZL patients. Overall design: Frozen cell suspensions obtained from diagnosed splenic marginal zone lymphomas were selected for injection in the 10X chromium device for droplet-based RNA extraction, reverse transcription and cDNA amplification. Libraries were made with the Chromium i7 sample index plate (GENO55823). Sequencing was performed on a NextSeq 550 (Illumina).