Single-cell RNA sequencing analysis of mouse cardiac tissues with myocardial infarction

To investigate the transcriotome alteration in myocardial infarction (MI)-associated cells, we performed RNA sequcening analysis with single cells derived from mouse infarcted cardiac tissues or normal left ventricle (LV). Particularly, cardiac endothelial cells (ECs) were traced using a Cdh5-Cre;LSL-tdTomatom system. Overall design: Myocardial infarction (MI) was induced Cdh5-CreERT2;Rosa-LSL-tdTomato mice. 0, 1, 2, 3, 8 weeks after the surgery, infarcted tissues or normal left ventricle (LV) were harvested. Single-cell suspensions were analyzed by RNA sequencing.