LncRNA/mRNA expression profiling for diabetic and reversal rat retina samples

We employed LncRNA/mRNA expression profiling as a discovery platform identify aberrantly expressed LncRNAs that fail to reverse back after hyperglycemia is terminated. Using rat model of diabetic retinopathy- metabolic memory, LncRNA array was performed in the retina of rats diabetic for eight months, or in poor glycemic control for four months followed by good glycemic control for four additional months. Expression of selected LncRNAs and their associated genes from these profiles were confirmed by quantitative real-time PCR. Differential expression profiling for LncRNA/mRNA was measured for normal, diabetic (8 months) and reversal (diabetic for four months followed by good glycemic controlfor four months) rat retina samples. Each group had 6-7 animals.