Taxonomic reassessment of genomes from a divergent population of Streptococcus suis by average nucleotide identity analysis

This dataset provides genomic-level re-identification and taxonomic
reassessment of Streptococcus suis out-population strains based on average
nucleotide identity (ANI), 16S rRNA gene comparisons, and other
phylogenetic markers. The dataset includes an Excel workbook with eight
sheets (Sheet A–H), which together constitute Supplementary Table S1. This
table contains detailed information on the studied strains, including
their disease association, sequence alignment results (identity, coverage,
and length) of 16S rRNA genes against both the S. suis type strain S735
and a database of prokaryotic type strains, ANI and AAI values compared to
S735, accession numbers for short-read and long-read data, genome assembly
IDs, phylogenetic lineage within the S. suis tree, group classification
within the out-population, and cluster medoid status indicating
representative strains. Through comparative genomic analysis, this study
established an ANI threshold of 93.17% for defining authentic S. suis,
revealing that all 645 genomes from the out-population fell below this
cutoff and thus did not belong to S. suis. Further pairwise ANI comparison
identified 18 distinct clusters among these strains, leading to the
proposal of 12 novel Streptococcus species and the reclassification of six
as known species. This dataset serves as a valuable resource for
researchers studying streptococcal taxonomy, zoonotic pathogens, and
bacterial evolution. It enables further investigations into genomic
diversity, species delineation, and the evolutionary relationships within
the genus Streptococcus.