Single cell RNA-sequencing reveals placenta cellular heterogeneity in adverse pregnancy

The placenta is a highly heterogeneous organ and is closely related to adverse pregnancy. The previous bulk sequencing of whole tissue could not show the characteristics of individual cells and the interactions between cells. Here, we select the placental tissues of the gestational diabetes group(GDM), preeclampsia group(PE), advanced age group(GL) and normal control group for single-cell sequencing in order to explain the mechanism of related diseases in more depth.nated spatial and temporal regulation of gene expression in the murine hindlimb determines the identity of mesenchymal progenitors and the development of diversity of musculoskeletal tissues they form. Hindlimb development has historically been studied with lineage tracing of individual genes selected a priori, or at the bulk tissue level, which does not allow for the determination of single cell transcriptional programs yielding mature cell types and tissues. To identify the cellular trajectories of lineage specification during limb bud development, we used single cell mRNA sequencing (scRNA-seq) to profile the developing murine hindlimb.