For sEVs-educating mice experiments, each mouse was injected with 20μg of CRC-sEVs (SW620, LoVo) or normal colon cell-sEVs via tail vein every other day for 21 days. After 2 weeks, all mice were given
To reveal immune microenvironment heterogeneity at single-cell resolution, we performed single cell transcriptome analysis of CD45 immune cells from Ythdf1-KO and WT tumors.