4T1_series_murine_mammary_cancer_cell_line_exome_sequencing

The existing 4T1 series of cell lines demonstrate a trend from least to most metastatic cell line with regards to phenotype. Microarray analyses of the 4T1 series cell lines and cell line-derived mammary tumours have been previously published. From such data gene expression patterns associated with individual steps of mammary tumour metastasis have been identified but exome sequencing of the 4T1 series has not been published before. Analysis of the exome capture of each cell line in the 4T1 series should in theory identify mutations relevant to the metastatic ability of each cell line, which could in turn explain the associated phenotype. Identifying causal mutations using this method would lead to a greater understanding of the genes, be it with an oncogenic or suppressive role, involved in the various stages of the metastatic cascade. The clinical relevance would include the elucidation of potential therapeutic targets.