Single cell RNA sequencing analysis of the center region of healing skin at wound day 11 in wild type mouse

We have applied single_x0002_cell RNA sequencing analysis to investigate keratinocyte populations and their specific gene expression on the day of would closure (post wounding day 11-12) at the center of re-epithelialized mouse wound site (1 cm2 of whole skin was removed, multiple pooled mice, n = 10). Viable cells from single cell suspension were selected by sorting and single cell library was prepared by 10x Genomics and sequenced by Illumina NovaSeq 6000. Downstream bioinformatic analysis of single cell RNAseq was performed by using Seurat package in R. Clusters of cells were denoted according to their identity. Keratinocytes were identified from the entire sample of cells using known keratinocyte markers. Specific gene expression over the keratinocytes were visulaized by heatmap. Overlap expression of Cd14, Tlr3, Krt15, Krt19 and Rara at the day of wound closure supports morphogenesis. Overall design: Single sample was analyzed to investigation of keratinocyte gene expression on the day of would closure (post wounding day 11-12) at the center of re-epithelialized mouse wound site