Epigenomic profiling of human Axioloids with CUT&Tag

We have established a pluripotent stem cell (PSC)-based 3D model of human axial development, which recapitulates in vitro key features of the somitogenesis process including axial elongation, segmentation and sequential formation of epithelial somites with proper rostrocaudal pattering, and traveling wave-like gene expression dynamics associated with the segmentation clock. Initial molecular and functional analysis of these axially-organized embryonic tail-like structures, which we termed Axioloids, revealed the spatiotemporally controlled self-organization of somitogenesis associated human mesodermal cell populations. In order to gain a deeper understanding of this model, we performed CUT&Tag experiments using anti-H3K4me3 and anti-H3K27me3 antibodies to analyze the epigenomic landscapes in the Axioloids. CUT&Tag profiling for active (H3K4me3) and repressive (H3K27me3) epigenomic landscapes in human Axioloids