RNA sequencing analysis of decidua tissues from unexplained recurrent spontaneous abortion patients and controls with induced abortions

Recurrent spontaneous abortion (RSA), defined as the failure of two or more consecutive clinical pregnancies before 20 weeks of gestation, affects approximately 1% of couples attempting to conceive. However, the underlying mechanisms of 50% of cases are unknown. The decidua plays a crucial role during pregnancy, which can provide a delicate balance between immune tolerance and defense to maintain the pregnancy.We performed RNA-seq to identify gene expression alterations in the deciduas of RSA patients and controls. RNA-seq was done using TruSeq Stranded mRNA Library Preparation. Briefly, intact RNA was fragmented, end repaired, adapter ligation and PCR amplified following illumina protocol. Libraries were sequencing by illumins Hiseq 2000. After quality control, sequence data were processed with STAR to generate read alignments with hg19. Raw read counts for annotated genes were obtained with featureCounts with default settings, normalized and analyzed using DEseq2. Overall design: Intact RNA isolated from 6 human decidua tissues was fragmented, end repaired, adapter ligation and PCR amplified following illumina protocol. Libraries were sequencing by illumins Hiseq 2000. UPDATE: [Jan-29-2026] The titles of GSM3119483-GSM3119485 were changed from 'decidua_induced abortion' to 'decidua_recurrent spontaneous abortion', and the titles of GSM3119486-GSM3119488 were changed from 'decidua_recurrent spontaneous abortion' to 'decidua_induced abortion'. The raw data and processed data associated with each GSM did not change.