Generation and Characterization of a variant of Thermostable Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV-RT) Enzyme

Reverse Transcriptase (RT) enzyme has been utilized extensively, especially in the Polymerase Chain Reaction (PCR). Many viruses that cause infections worldwide contain RNA (Ribonucleic acid), and for their molecular detection, it is essential to convert the RNA to DNA using RT. This study aimed to create and characterize a thermostable Moloney Murine Reverse Transcriptase (MMLV-RT) enzyme using consensus sequences from the latest MMLV-RT sequence database