Urinary Cell Transcriptomics and Acute Rejection in Human Kidney Allografts

RNA-sequencing (RNA-Seq) is a precise tool to analyze global transcriptional changes, develop biomarkers, and decipher pathogenic mechanisms. We performed RNA-Seq of urinary cells to investigate gene expression patterns and pathways and whether urinary cell mRNA transcriptional profiles are enriched for biopsy transcriptional profiles. We performed RNA-Seq of 57 urinary cell pellets of kidney allograft recipients obtained at the time of biopsy diagnosis of T cell mediated rejection (N=22), antibody mediated rejection (N=8), or Normal/nonspecific changes/no rejection (N=27)