Directed Evolution of Escherichia coli Nissel 1917 to Utilize Allulose as Sole Carbon Source

We obtained a group of Escherichia coli Nissle 1917 (EcN) mutants, a commonly used probiotic which can consume D-allulose as sole carbon source and produce beneficials at the same time, using multiple directed evolution manners combining Adaptive Laboratory Evolution (ALE) and Fluorescence-Activated Droplet Sorting (FADS). The genome of three obtain EcN mutants were extracted and constructed to DNA libraries, which were sequenced on the illumina NovaSeq 6000 platform.