Mean quantification cycle (Cq) values of viral genome detection via EBLV-1 and EBLV-2 specific real-time RT-PCR.
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RABV: Rabies virus;;EBLV: European Bat Lyssavirus;neg.: negative control; –: not applicable; #: cross–reactivitiy with other Lyssavirus species; ?: inconclusive based on curve shape; dilution series (0), (I), (II), (III); 100, 10−1, 10−2, 10−3;*Hoffmann and Müller personal communication; ts: two-step systems; no duplicates for assays M1 and M2; all laboratories except J1 used separate species-specific real-time PCRs to detect EBLV-1 or -2.
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2015-12-02



