Transcriptome analysis upon Nipbl, Brd2 and Brd4 knockdown in P19 cells. Transcriptome analysis upon Nipbl, Brd2 and Brd4 knockdown in P19 cells

Mutations in NIPBL are the major cause of Cornelia de Lange Syndrome (CdLS). NIPBL is the cohesin loading factor and has recently been associated with the BET (Bromodomains and Extra Terminal (ET) domain) proteins BRD2 and BRD4. Related to this, a CdLS-like phenotype has been described associated to BRD4 mutations. To understand the relationship between NIPBL and BET proteins, we have performed RNA-Seq expression analysis following depletion of the different proteins in mouse P19 teratocarcinoma cells. Results indicate that genes regulated by NIPBL largely overlap with those regulated by BRD4 but not with those regulated by BRD2. Overall design: mRNA profiles of P19 mouse teratocarcinoma cells treated with siControl, siNipbl, esiBrd2 or esiBrd4 were generated by deep sequencing, in duplicates, using Illumina Nexseq.