Mouse lung and brain LCM-ATACseq

In situ transposition followed by laser capture microdissection was applied to lung and brain mouse tissue sections to demonstrate feasibility of LCM-based spatial chromatin accessibility analysis of regions of interest. Overall design: Fresh frozen lung and brain tissue sections from C57BL/6J WT mice were tagmented in situ to assess chromatin accessibility. LCM was employed to collect a mini bulk of randomly captured nuclei. Two mini-bulk sizes were investigated: 50 single-captured nuclei or 10 larger captured-areas composed each of roughly 20 nuclei, for a total of ~200 nuclei. This was applied both on brain and lung samples, at least in technical duplicates.