ExtFig6B_RACE_STOML2_R1_LG388_annotated.pdf

HEK293T ishXrn1 cells were treated with doxycycline for 3-4 days to induce knock down of Xrn1, then transfected with a luciferase reporter with a 15 bp inserted sequence from the STOML2 gene, wild-type or containing mutations in GC basepairs to disrupt the hairpin structure, or contaning mutations in GC basepairs that preserve the hairpin structure. They were infected 24 hours later with influenza A/Puerto Rico/8/1984 (H1N1), either wild-type or PA-X deficient (deltaX)  or mock infected. RNA was collected 24 hrs later and analyze by 5' RACE using a primer that binds to luciferase sequences.