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Additional file 2: Table S1. of Hybrid de novo genome assembly and centromere characterization of the gray mouse lemur (Microcebus murinus)

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Quality assessment and assembly statistics of iterative genome assemblies of Microcebus murinus. Table S2. BioNano Genomics in silico physical map production, conflict resolution, and hybrid scaffolding statistics for two M. murinus assemblies (see Fig. 1). Table S3. Hi-C mapping and Lachesis assembly statistics. Statistics for mapping of Hi-C Illumina reads to BioNano generated assemblies are shown. Mapping percentages are based on a total of 2,094,030,784 sequenced Hi-C Illumina reads. Lachesis generated clustering, ordering, and orienting assembly statistics based on the Hi-C mappings for the two rounds of Lachesis are also shown. Table S4. Pilon error correction. The number of bases and indels corrected by Pilon after the application of PBJelly are shown. The length of corrected indel bases are also shown. Table S5. GATK estimates of SNP and indel error rates. Homozygous alternative (non-reference) alleles provide estimates of base and indel error rates in the assembly. A caveat of this is that assembled Illumina reads and PacBio reads are from different individuals. Table S6. Comparative cytogenetic data showing homologous chromosomes between human and mouse lemur. Data summarized from [29], with addition of the X chromosome (inter intercalated, min minute, prox proximal, ter terminal). Table S7. Mouse lemur chromosome assignments to the 33 Lachesis groups identified herein (see Fig. 4 and Additional file 4: Figure S3). Bold font identifies the 23 chromosomes that are supported by comparative cytogenetic data ([29]; Fig. 4, Additional file 2: Table S6). The remaining 10 chromosomes are putative assignments pending FISH confirmation. Table S8. Microcebus murinus samples used for genetic data presented herein. Mitochondrial haplotype identification for each sample is based on phylogenetic analyses of 1140 bp of the cytochrome-b gene. BCM Baylor College of Medicine, DLC Duke Lemur Center. (XLSX 36 kb)
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2017-11-17
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