Hypoxia-induced translational profiles of murine bone marrow derived macrophages (BMDM)

We report changes in total and translated poly(A) RNA in mouse bone marrow derived macrophages after exposure to hypoxia. We employed translating ribosome affinity purification (TRAP) to isolate polysomal RNA from mouse bone marrow derived macrophages after exposure to hypoxia Overall design: Total and translated poly(A) RNA samples were isolated from BMDM isolated from 3 conditional HuR-knockout (Csf1r-iCre.HuRfl/fl) and 3 control mice (Csf1r-iCre.HuR+/fl) expressing ribosomal protein L10a fused to EGFP. BMDM were differentiated in vitro and exposed to normoxia or hypoxia (1% O2) conditions for 18 h.