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N-acetylation of α-synuclein enhances synaptic vesicle clustering mediated by α-synuclein and lysophosphatidylcholine

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To characterize the accessibility of α-syn to LPC and DOPS, we performed cross-linking experiments coupled with mass spectrometry (XL-MS). We mixed 15N-labeled and unlabeled Ac-α-syn at a 1:1 ratio and selected the cross-linked fragments containing both 15N-labeled and unlabeled Ac-α-syn to map the intermolecular cross-linking pattern.

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2024-12-26
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