Deep sequencing and methylation profiling of both treated and untreated primary and recurrent tumours confirms the heterogeneous and dynamic nature of glioblastoma

Combined targeted amplicon sequencing and DNA methylation analysis of a defined cohort of Adult Glioma patients was performed on primary and matching recurrent tumour samples to investigate tumour evolution. 21 patients from which primary and matching recurrent tumour samples were analysed for DNA methylation using Illumina HumanMethylation450K bead array. Samples from 6 patients were performed in replicate.