Expression data of PRDM1α or empty vector-transduced NK92 or KHYG1 cell lines. Expression data of PRDM1α or empty vector-transduced NK92 or KHYG1 cell lines

CD25 (IL2RA) is a subunit of IL2 receptor complex, which determines the sensitivity of the receptor to IL2. Here, we investigated whether PRDM1, a transcriptional repressor implicated as a tumor suppressor in NK cell malignancies, directly represses CD25 (IL2RA). Using ChIP-seq, we identified a direct binding site of PRDM1 within 1st intron of CD25 (IL2RA) in activated primary human NK cells. We used DNA microarrays on two PRDM1α transduced NK cell lines (i.e. NK92 and KHYG1) to address whether PRDM1 transcriptionally represses CD25. Overall design: Exponentially growing NK92 and KHYG1 cell lines were transduced with PRDM1α-PMIG or empty PMIG retroviral constructs. GFP+ cells were sorted with FACS 48h post-transduction. Total RNA was purified from sorted cells and subjected to DNA microarray.