Kinetic values of wild type PG8fn and its mutants a.
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a The kinetic values are shown as means ± standard deviations (n = 3).b The kcat values were calculated by considering the enzyme to be a monomeric form.c △(△G) = −RT·ln[(kcat/Km)mut/(kcat/Km)wt], where (kcat/Km)mut and (kcat/Km)wt are the kcat/Km ratios of the mutant and wild type enzyme, respectively, R is the ideal gas constant, and T is the temperature in Kelvin.Kinetic values of wild type PG8fn and its mutants a.
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2015-12-03
