Identify key factors regulated by transcription factor Cebpd in the kidney during heart regeneration in zebrafish

In previous studies, we identified and demonstrated that a 1.2kb cebpd downstream element (CEN) is capable and neccessary for the induction of cebpd in the kidney after cardiac injury. The goal of this study is to unveal the Cebpd regulated responses of kidney to cardiac regeneration in zebrafish. we profiled responses by kidney in cebpd mutant and enhancer (CEN) mutant fish zebrafish to massive injuries of the heart. Overall design: cebpd+/+ and cebpd-/- kidney RNA-seq profiling after cardiac injury; CEN+/+ and CEN-/- kidney RNA-seq profiling after cardiac injury;