RNA-seq of bone marrow neutrophil treated PGE2 or Vehicle. RNA-seq of bone marrow neutrophil treated PGE2 or Vehicle

Purpose: Investigate the neutrophils could exspress different mRNA de novo from PGE2 stimulation. Methods: Isolated bone marrow neutrophils with Percoll gradient are treated with 10 μM of PGE2 for 24 hours. Results: Qualified sequence reads per sample to the mouse genome (mm10) with Bowtie2 or STAR were processed with StringTie and identified genes. Conclusion : 1,359 genes were significantly different in PGE2 treated bone marrow neutrophils from vehicle treated ones. Overall design: RNA-seq analysis of neutrophils isolated from bone marrow treated 10μM PGE2 or DMSO vehicle for 24 hours. 3 biological replicates.