Design matrix of outcomes from a Plackett-Burman experimental design for the screening of significant nutrient factors.

N1 to N11 represent the nutrient factors in the following order with the low and high uncoded values in brackets: casein (1&7 g/L), peptone (1&7 g/L), lactose (1&5 g/L), sucrose (1&5 g/L), fructose (1&5 g/L), maltose (1&5 g/L), nano3 (0.5&5 g/L), glucose (1&5 g/L), L-glutamine (2&6 g/L), yeast extract (0.5&2g/L) and beef extract (0.5&2 g/L). E.A = Enzyme activity IU/mL, P.E.A = Predicted Enzyme Activity IU/mL.